The development of live attenuated vaccines involves several steps: 1. Isolation of the Pathogen: The pathogen causing the disease is isolated. 2. Attenuation: The pathogen is weakened through various methods, such as passing it through different cell cultures or animal embryos. 3. Testing: The attenuated pathogen is tested in pre-clinical and clinical trials to ensure it is safe and effective. 4. Regulation and Approval: Regulatory bodies review the data and approve the vaccine for public use.
